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KMID : 1134820070360080989
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Journal of the Korean Society of Food Science and Nutrition
2007 Volume.36 No. 8 p.989 ~ p.994
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Inhibitory Effects of Sasa borealis Leaves Extracts on Carbohydrate Digestive Enzymes and Postprandial Hyperglycemia
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Hwang Ji-Young
Han Ji-Sook
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Abstract
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This study was designed to investigate whether Sasa borealis leaves extracts (SLE) may inhibit yeast α?glucosidase and α?amylase activities and postprandial hyperglycemia in STZ?induced diabetic mice. Freeze?dried SLE was extracted with 70% methanol and followed by a sequential fractionation with dicholoromethan, ethylacetate, butanol, and water. Both ethylacetate and butanol fractions showed high inhibitory activities against the α?glucosidase and α?amylase enzymes. The IC?? of ethylacetate and butanol fractions against α?glucosidase were 0.54 and 0.63 ㎎/mL, respectively, indicating a greater inhibition effect than acarbose (0.68 ㎎/mL) (p＜0.05). Likewise, the two fractions exhibited a smaller IC?? against α?amylase, compared with acarbose (p＜0.05). However, the yield of ethylacetate fraction of SLE was relatively small. Postprandial blood glucose testing of normal mice and STZ?induced diabetic mice by starch soln. loading (2 g/㎏ B.W.) showed that postprandial blood glucose level at 30, 60, and 120 min were markedly decreased by single oral administration of SLE butanol fraction (200 ㎎/㎏ B.W.) in both normal (p＜0.01) and diabetic mice (p＜0.01). Furthermore, the incremental area under the curve (AUC) was significantly lowered via SLE administration (5,745 versus 12,435 ㎎ㆍmim/dL) in the diabetic mice (p＜0.01). The incremental AUC in normal mice corroborated the hypoglycemic effect of SLE (p＜0.01) found in the diabetic mice. These results suggest that SLE may delay carbohydrate digestion and thus glucose absorption. In addition, SLE may have the potential to prevent and treat diabetes via its ability on lowering postprandial hyperglycemia.
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KEYWORD
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Sasa borealis leaves, α-glucosidase, α-amylase, anti-hyperglycemia
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